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میڤان فهمی سامی محمد

میڤان فهمی سامی محمد

  • Academic title: Lecturer
  • Certification / qualification: Ph.D.
  • Working at: Department of Biology
  • Email: mehvan.jacksi@uoz.edu.krd
  • Working address: Presidency - A / Floor 1 / 24 (Office)
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Language Proficiency
Kurdish Native
English B2
Arabic C1
Kurdish Native Language
Arabic C2
English B2
Experience name
* Research interest: rnMolecular Medicine, Molecular Biology, Medical Physiology, Oncology, Biotechnology, Cell Biology, Biochemistry, and Genetics.
* Medical and Molecular techniques and methods: rno PCR: qPCR, RT-PCR, and conventional PCR, PCR optimization. rno Immunoblotting: Western blotting, ELISA, dot-blot. rno Immunoprecipitation: Chromatin immunoprecipitation (Chip-Seq and Chip-qPCR), RNA immunoprecipitation (RIP-seq and RIP-qPCR), Co-immunoprecipitation (Co-IP) followed by WB or Mass spectrometry, and Crosslinking immunoprecipitation followed by sequencing (CLIP-seq). rno Immunostaining: Immunocytochemistry rno Cell and Tissue culture: cell culture of adherent and suspension cells, cell passages, and cryopreservation. rno Assays: Wound healing, migration, invasion, and proliferation assay. rno Primer and Probe designing rno Extraction methods: Nucleic acid extraction (Eukaryotic, prokaryotic, and gel) and protein extraction (Cytoplasmic and Nuclear) rno Protein determination: BCA assay, Bradford assay, and Qubit 4 based determination) rno Transfection (lipofectamine and electroporation) and transformation (heat shock). rno Gene knockdown (RNA silencing) rno Gene knock-in (plasmid insertion) rno Site-directed mutagenesis (generate mutation) rno Electrophoresis: PAGE, Agarose Gel Electrophoresis. rno Staining gel and blots: Silver staining, Ponsue Red, BradFord, and Coomassie Blue. rno Microscope: Confocal Microscope (nondependent), widefield fluorescence microscope, Light Microscope, Dark-field Microscopy, Dissecting Microscope. rno Others: Sonication (Eukaryotic and Prokaryotic), Spectrophotometer, Nanodrop, Plate reader, Autoclaving, ...etc.
* Medical and Molecular software: rno Creation of Image, Sketch and Diagram: Biorender, Adobe Photoshop, IBS, GraphPad prism, and Excel.rno Statistical analysis: GraphPad prismrno Sequencing analysis: KEGG, Venn graph, Reactome, and Heatmap. rno Image analysis software: ImageLab (Western blot), ImageJ, GraphPad, and PhotoScape... etc. rno Gene manipulation and Primer design software: Snap gene and Serial Clonerrno PCR software: QuantStudio™ Design & Analysis Software (3, 5, and 6 pro) and Step One Software v2.3rno Confocal Microscope: ZENrno Quality and quantity detection software: Nanodrop and Plate reader softwarerno Referencing software: Mendeley and RefWorks. rno Excel: for Analyzing NGS data, qPCR fold enrichment, RIP-seq, Chip-seq fold enrichment.
* Medical and Biological Website:rno Signaling pathway analysis: KEGG and Reactome rno Alignment Tools: NCBI nucleic acid BLAST and protein BLASTrno Oligo (primer and probe) designing tools and calculation for PCR, qPCR, cloning, and Sanger sequencing: Primer-BLAST, Primer3, ThermoCalc. and RC, OligoPerfect.rno Molarity, Mass, OD, and dilution calculation: NEBio and GraphPadrno RNA Structure Prediction: ViennaaRNA Web Services.rno Protein-Protein interaction and connection: STRINGrno Bioinformatics: SMS, Venn graph, dosztanyi web, Phobius, Biobam, MPI, GOrilla, RCSB, PhosphositePlus, and IUpred.rno Sketch and Diagram design website: Biorender
Award name Date Organization
The role of EZH2 in TKS4-deficient colorectal carcinoma cells 2024-08-01 Hungarian Biochemical Society
A Beginner's guide to DNA sequencing 2023-10-01 Novogene
The role of Ezh2 in the Tks4 deficient cells inducing migration, invasion, and EMT-related processes 2023-03-01 Hungarian Molecular Life Sciences (2023)
A Beginner's Guide to DNA Sequencing Recent Advances 2022-10-01 Novogene
Next generation sequencing and its Clinical Applications 2022-09-01 Novogene
ncRNA-seq results explained: What you can explore with non-coding RNA data 2022-07-01 Novogene
Next generation sequencing solutions for transcriptomics and epigenetics research 2022-05-01 Novogene
A Beginner's guide to DNA-seq-Bioinformatic analysis 2022-04-01 Novogene
The role of Ezh2 and Tks4 in EMT-related processes 2021-11-01 Hungarian Molecular Life Sciences 2021
The complete guide to research paper writing 2021-04-01 Research Graduate Institute
Certificate of teaching methods 2015-12-01 UOZ
Training course name Date Organizing organization
Certificate of Teaching Methods 2015-09-15 Teaching Methods for higher education
Thesis title Actions
EXPRESSIONAL PROFILING OF LINC00663 RNA IN VARIOUS HUMAN TISSUES AND CANCER CELL LINES
Conference name Actions
The role of EZH2 in TKS4-deficient colorectal carcinoma cells
Investigating the role of RNA binding in the cellular function of KMT2D
The role of Ezh2 in the Tks4 deficient cells inducing migration, invasion, and EMT-related processes
The role of Ezh2 and Tks4 in EMT-related processes
The RBR-polyQ Region Of KMT2D: Unique Sequence-Specific Function
Kanser hücrelerinde non-coding LINC00663 RNA’nın yeni splice varyantlarının belirlenmesi ve bu splice varyantlarınpotansiyel ikincil yapılarının gösterilmesi
Çeşitli İnsan Dokuları ve Hücre Hatlarında non-coding LINC00663 RNA’nın Gen İfade Profili
Workshop title Actions
E-JOURNAL OF UNIVERSITY OF ZAKHO “A PEER REVIEW SYSTEM”
Article title Actions
Morphological Changes Induced by TKS4 Deficiency Can Be Reversed by EZH2 Inhibition in Colorectal Carcinoma Cells
KMT2D preferentially binds mRNAs of the genes it regulates, suggesting a role in RNA processing
Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
A novel variable exonic region and differential expression of LINC00663 non-coding RNA in various cancer cell lines and normal human tissue samples